Fig. 2

Cytopathic and adhesion assays of E. histolytica and E. moshkovskii with mouse sera. CHO cells were incubated with E. histolytica and E. moshkovskii trophozoite cells in the presence of mouse sera immunized with E. histolytica (Eh) or E. moshkovskii (Em) alone, mixed of E. histolytica and E. moshkovskii followed by E. histolytica (Eh + Em > Eh), and the mixed cells followed by E. moshkovskii (Eh + Em > Em). The effect of mouse sera on cytopathic activity and adhesion of E. histolytica and E. moshkovskii to CHO cells were determined by cell staining with propidium iodide (PI) and counting of PI positive and adhered CHO cells, respectively. PC is amoeba and CHO mixture without serum. B4 and B5 represent sera collected 2 weeks and after 4 times of trophozoite-cell immunization. Pre-immunized serum of each mouse was use as a negative control. The results are presented as percent cell death with 95% confidence interval from three independent experiments. F-values refer to ANOVA across all three challenges, asterisks indicate significant differences between challenges (Tukey’s post-hoc comparison), **P < 0.01 and *P < 0.05