Fig. 4

Proportion of Entamoeba species-specific mAbs and their specificities to cellular components. Mice were immunized with trophozoite cells of E. histolytica and E. moshkovskii followed by mixed cells of E. histolytica and E. moshkovskii, Eh > Eh + Em and Em > Eh + Em (a), respectively. The total antibody responses of the immunized mice were determined by ELISA using total lysate proteins of trophozoite cells of E. histolytica (solid circle) and E. moshkovskii (solid square) trophozoite cells. Proportions of mAbs specific to E. histolytica (Anti-Eh), E. moshkovskii (Anti-Em) and cross-species (Anti-Ehm, and Anti-Ehd, Anti-Ehmd) and their specificity toward cytoplasmic, membrane and cytoskeletal compartments of E. histolytica and E. moshkovskii are represented as % of mAbs (b, c)