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Table 1 Reverse line blot hybridization probe sequences with a C6 amino linker at the 5′ end

From: “Tekenscanner”: a novel smartphone application for companion animal owners and veterinarians to engage in tick and tick-borne pathogen surveillance in the Netherlands

Probe

Sequence (5′–3′)

Reference

Ehrlichia/Anaplasma catch-all

GGGGGAAAGATTTATCGCTA

[21]

Anaplasma phagocytophilum (1)

GCTATRAAGAATARTTAGTGG

[36]

Anaplasma phagocytophilum (2)

TTGCTATRRAGAATARTTAGTGG

[37, 38]

Anaplasma platys (1)

CGGATTTTTGTCGTAGCTTGCTATGAT

[36]

Anaplasma platys (2)

GTCGTAGCTTGCTATGATA

[39]

Ehrlichia canis (1)

TATAGCECTCTGGCGGAAATTGGTTAG

[36]

Ehrlichia canis (2)

TCTGGCTATAGGAAATTGTTA

[37]

Ehrlichia ewingii

TTCCTAAATAGTCTCTGACTATTT

[40]

“Ca. Neoehrlichia mikurensis”

GCTGTAGTTTACTATGGGTA

[37]

Theileria/Babesia catch-all

TAATGGTTAATAGGARCRGTTG

[20]

Babesia catch-all (1)

ATTAGAGTGTTTCAAGCAGAC

[38]

Babesia catch-all (2)

ACTAGAGTGTTTCAAACAGGC

[38]

Babesia caballi

GTGTTTATCGCAGACTTTTGT

[20]

Babesia canis (1)

GGTTGGTTATTTCGTTTTCGC

This study

Babesia canis (2)

TGGTTGGTTATTTCGTTTTCG

[38]

Babesia divergens

ACTRATGTCGAGATTGCAC

[16]

Babesia gibsoni (1)

CTGCGTTGCCCGACTCG

[36]

Babesia gibsoni (2)

TACTTGCCTTGTCTGGTTT

[38]

Babesia microti (1)

GCTTYCGAGCGTTWTTTTATTG

This study

Babesia microti (2)

GRCTTGGCATCWTCTGGA

[38]

Babesia rodhaini

TGTGGATTAGTGCGCAAG

[38]

Babesia rossi

CGGTTTGTTGCCTTTGTG

[41]

Babesia venatorum

CGATTTCGCTTTTGGGATT

[12]

Babesia vogeli

AGCGTGTTCGAGTTTGCC

[41]

Babesia vulpes (1)

CCGAACGTAATTTTATTGATTTG

[12]

Babesia vulpes (2)

CTTATCATTAATTTCGCTTCCGAACG

[36]

Borrelia burgdorferi (s.l.)

CTTCCATCTCTAYTTTGCCAAT

[42]

Borrelia burgdorferi (s.s.)

AACACCAATATTTAAAAAACATAA

[19]

Borrelia afzelii

AACATTTAAAAAATAAATTCAAGG

[19]

Borrelia bissetti/Borrelia carolinensis

CACTAACATTTAAAAAATATAAAATAAAAT

[42]

Borrelia garinii (1)

AAAATCAATGTTTAAAGTATAAAAT

[43]

Borrelia garinii (2)

AACATGAACATCTAAAAACATAAA

This study

Borrelia lusitaniae

TTTTTAAATCAAACATTCAAAAAAAT

[42]

Borrelia miyamotoi

AGCACAACAGGAGGGAGTTCAAGC

[44]

Borrelia spielmanii

GTCAATATCTATTTTCTTTTTTATG

[42]

Borrelia valaisiana (1)

CATTAAAAAAATATAAAAAATAAATTTAAGG

[19]

Borrelia valaisiana (2)

CATGTCAATATCTATTTTATTTTTTACATTA

[42]

Rickettsia catch-all

TTTAGAAATAAAAGCTAATACCG

[45]

Rickettsia conorii

CTTGCTCCAGTTAGTTAGT

[45]

Rickettsia helvetica

GCTAATACCATATATTCTCTATG

[45]

Rickettsia massiliae

TGGGGCTTGCTCTAATTAGT

[46]

Rickettsia raoultii

CTAATACCGCATATTCTCTACG

[12]

“Ca. Midichloria mitochondria”

GCGAAATAACAGTTGGAAGCAAT

[18]

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Parasites & Vectors

ISSN: 1756-3305

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