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Fig. 1 | Parasites & Vectors

Fig. 1

From: Hepatocellular carcinoma-associated antigen 59 of Haemonchus contortus modulates the functions of PBMCs and the differentiation and maturation of monocyte-derived dendritic cells of goats in vitro

Fig. 1

Expression, purification and Western blot analysis of rHCA59 protein. Lane M: standard protein molecular weight marker. a rHCA59 protein was induced with 1 mM IPTG. Lane 1: expression of recombinant protein rHCA59 vector before purification; Lane 2: purified rHCA59 protein. b Western blot of rHCA59 protein. Lane 1: recombinant protein HCA59 was recognized by goat anti-H. contortus sera; Lane 2: membrane incubated with normal goat sera (as control). c Western blot of total HcESPs. Lane 1: HcESPs were detected by rat anti-rHCA59 protein antibodies; Lane 2: membrane incubated with normal rat sera (as control)

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