Fig. 2From: Efficient genome engineering of Toxoplasma gondii using the TALEN techniqueIn vitro growth of tachyzoite-bradyzoite conversion and fluorescence microscopy analysis of transgenic T. gondii. HFF cells were infected with PRU/AAH2-eGFP tachyzoites and cultured in medium at pH 7.0 (a, b) or pH 8.2 (c, d). Transmitted light images (a, c) and green fluorescence images (b, d) were obtained by fluorescence microscopy. Scale-bars: 10 μm, 50 µmBack to article page