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Table 2 Diagnostic summary (blood smears, PCR and IFA)

From: A study on the long-term efficacy of Seresto® collars in preventing Babesia canis (Piana & Galli-Valerio, 1895) transmission to dogs by infected Dermacentor reticulatus (Fabricius, 1794) ticks

Animal ID

First tick challenge day

Last tick challenge day

Total no. of challenges

B. canis detected on blood smear (Day)

B. canis DNA detected by PCR (Day)

Antibodies detected by IFA (Day)b

4EF 726

28

56

2

70

70

84

4F2 719

28

56

2

66

66

84

CC2 D5A

28

56

2

68

68

84

DF4 FD4

28

56

2

65

65

84

DF6 939

28

84

3

93

93

112

DF6 AE1

28

28

1

42

39

56

DF7 98F

28

112

4

128

128

140

E19 4E8

28

28

1

37

37

56

B29 DBD

56

56

1

64

64

84

28D 55B

84

224

6

nd

nda

nd

5A1 364

84

84

1

93

93

112

5A6 66D

84

84

1

93

93

112

698 469

84

84

1

92

92

112

CC2 6AC

84

84

1

93

93

112

E18 24C

56

84

1

95

95

112

5A3 C4C

112

112

1

121

121

140

5A6 952

112

112

1

121

121

140

5A8 170

112

112

1

123

123

140

5D3 CA0

112

224

5

nd

nda

nd

689 702

112

112

1

121

121

140

698 04E

112

168

3

177

177

196

697 F9D

140

168

2

176

176

196

698 1A0

140

224

4

233

233

252

698 22F

140

224

4

nd

nda

nd

6D3 CE2

140

140

1

148

148

168

6D6 412

140

140

1

148

148

168

57C 00E

168

196

2

212

212

224

5A4 2FD

168

196

2

216

202

196

2A6 EA8

196

224

2

nd

nda

nd

2AD 0E4

196

224

2

nd

nda

nd

5BC D82

224

224

1

nd

nda

nd

B2F AAB

224

224

1

233

233

252

2A8 C98

28

224

8

nd

nda

nd

2AB E8B

28

224

8

nd

nda

nd

B2B 68D

28

224

8

nda

nd

CC3 20D

28

224

8

nd

nda

nd

DF5 A66

28

224

8

nd

nda

nd

DF7 5C1

28

224

8

nd

nda

nd

E17 329

28

224

8

nd

nda

nd

EA0 7C7

28

84

3

nd

nd

5A1 979

112

224

5

nda

nd

  1. aBlood collected and PCR performed at the end of the study assessment period
  2. bIFA testing performed on Days-7, 28, 56, 84, 112, 140, 168, 196, 224 and 252. Results only show Days where antibodies were detected or indicated “nd” where no antibodies were detected during any of the sequential tests
  3. Abbreviations: nd, not detected; –, not evaluated