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Table 2 Diagnostic summary (blood smears, PCR and IFA)

From: A study on the long-term efficacy of Seresto® collars in preventing Babesia canis (Piana & Galli-Valerio, 1895) transmission to dogs by infected Dermacentor reticulatus (Fabricius, 1794) ticks

Animal ID First tick challenge day Last tick challenge day Total no. of challenges B. canis detected on blood smear (Day) B. canis DNA detected by PCR (Day) Antibodies detected by IFA (Day)b
4EF 726 28 56 2 70 70 84
4F2 719 28 56 2 66 66 84
CC2 D5A 28 56 2 68 68 84
DF4 FD4 28 56 2 65 65 84
DF6 939 28 84 3 93 93 112
DF6 AE1 28 28 1 42 39 56
DF7 98F 28 112 4 128 128 140
E19 4E8 28 28 1 37 37 56
B29 DBD 56 56 1 64 64 84
28D 55B 84 224 6 nd nda nd
5A1 364 84 84 1 93 93 112
5A6 66D 84 84 1 93 93 112
698 469 84 84 1 92 92 112
CC2 6AC 84 84 1 93 93 112
E18 24C 56 84 1 95 95 112
5A3 C4C 112 112 1 121 121 140
5A6 952 112 112 1 121 121 140
5A8 170 112 112 1 123 123 140
5D3 CA0 112 224 5 nd nda nd
689 702 112 112 1 121 121 140
698 04E 112 168 3 177 177 196
697 F9D 140 168 2 176 176 196
698 1A0 140 224 4 233 233 252
698 22F 140 224 4 nd nda nd
6D3 CE2 140 140 1 148 148 168
6D6 412 140 140 1 148 148 168
57C 00E 168 196 2 212 212 224
5A4 2FD 168 196 2 216 202 196
2A6 EA8 196 224 2 nd nda nd
2AD 0E4 196 224 2 nd nda nd
5BC D82 224 224 1 nd nda nd
B2F AAB 224 224 1 233 233 252
2A8 C98 28 224 8 nd nda nd
2AB E8B 28 224 8 nd nda nd
B2B 68D 28 224 8 nda nd
CC3 20D 28 224 8 nd nda nd
DF5 A66 28 224 8 nd nda nd
DF7 5C1 28 224 8 nd nda nd
E17 329 28 224 8 nd nda nd
EA0 7C7 28 84 3 nd nd
5A1 979 112 224 5 nda nd
  1. aBlood collected and PCR performed at the end of the study assessment period
  2. bIFA testing performed on Days-7, 28, 56, 84, 112, 140, 168, 196, 224 and 252. Results only show Days where antibodies were detected or indicated “nd” where no antibodies were detected during any of the sequential tests
  3. Abbreviations: nd, not detected; –, not evaluated