Emerging feline vector-borne pathogens in Italy

Parasites & Vectors

Table 1 Pathogens, target genes, nucleotide sequences, primer melting temperatures, amplicon sizes (in base pairs) and references

Target species	Target gene	Primer sequence (5′–3′)	Tm (°C)	Amplicon size (bp)	Reference
Rickettsia spp.	gltA	GGGGGCCTGCTCACGGCGG	54	381	[40]
	gltA	ATTGCAAAAAGTACAGTGAACA	54	381	[40]
	ompB	GTAACCGGAAGTAATCGTTTCGTAA	54	511	[41]
		GCTTTATAACCAGCTAAACCACC	54	511
		GTTTAATACGTGCTGCTAACCAA	56	425
		GGTTTGGCCCATATACCATAAG	56	425
	ompA	ATGGCGAATATTTCTCCAAAA	50	532	[42]
	ompA	AGTGCAGCATTCGCTCCCCCT	50	532	[42]
Piroplasmida	18S rRNA	AATACCCAATCCTGACACAGGG	57	408	[43]
Piroplasmida	18S rRNA	TTAAATACGAATGCCCCCAAC	57	408	[43]
Leishmania spp.	18S rRNA	GGTTCCTTTCCTGATTTACG	60	603	[37]
		GGCCGGTAAAGGCCGAATAG		603	[37]
		TCCCATCGCAACCTCGGTT		358	[44]
		AAAGCGGGCGCGGTGCTG		358	[44]

ISSN: 1756-3305