Tissue-specific signatures in tick cell line MS profiles

Parasites & Vectors

Table 3 Assigned characteristic peaks in the MS profiles of Ixodes spp. tick cell lines to proteins identified in the corresponding extracts

m/z	Accession^a	Description^b	Species	MW (Da)^c	Tick cell line^d	Tick organ^e
6154	B7QEH2	Conserved hypothetical protein (F1-ATPase epsilon superfamily)	I. scapularis	6160	All	All
6656	Q4PM47	Ribosomal protein S29	I. scapularis	6653	IRE/CTVM19; ISE6; ISE18	–
8565	A0A131Y5V6	Putative cytoplasmic cystatin	I. ricinus	8612	All	All
9182	V5H067	Putative ciboulot_ partial	I. ricinus	9189	IRE/CTVM19; ISE18	SG
9773	A0A0K8RPM6	Putative ubiquitin/40s ribosomal protein s27a fusion	I. ricinus	9696	All	–
9971	V5H1M0	Putative ATP synthase e chain Rhipicephalus sanguineus ATP synthase e chain	I. ricinus	9964	IRE11; IRE/CTVM19; IRE/CTVM20	Ov
9971	Q4PM77	ATP synthase E chain	I. scapularis	9964	ISE6; ISE18	Ov
11515	A0A0K8RQR8	Putative histone h4_partial	I. ricinus	11488	IRE/CTVM20	–
13312	A0A0K8RIJ1	Putative histone 2a	I. ricinus	13355	IRE11; IRE/CTVM19; IRE/CTVM20	SG, MT
13312	B7PSI5	Histone 2A	I. scapularis	13355	ISE6; ISE18	SG, MT
13521	A0A090XDF4	Putative glycine-rich secreted protein 95	I. ricinus	13504	IRE/CTVM19	G, MT
13775	A0A0K8RB64	Putative histone h2b	I. ricinus	13810	IRE11; IRE/CTVM19; IRE/CTVM20	SG, Ov, MT
13775	Q4PM63	Histone 2B	I. scapularis	13810	ISE6; ISE18	SG, Ov, MT
15364	A0A0K8R3K9	Putative histones h3 and h4	I. ricinus	15318	IRE/CTVM19	–

^aAccession number refers to the sequence retrieved from the UniProt protein database
^bDescription of the respective protein accession. Protein homologs were assigned to unknown proteins via BLAST search
^cTheoretical molecular weight
^dPresence of a peak in the MS profiles of tick cell lines
^ePresence of a peak in the MS profiles of tick organs
Abbreviations: G, gut; SG, salivary gland; Ov, ovary; MT, Malpighian tubules

ISSN: 1756-3305