Fig. 1

Tick feeding parameters in tHRF immunized mice. a, d IgG titers in OVA- and tHRF-immunized mice. The sera were tested at the beginning of the assay (IgG d0), after the second boost (IgG d28) and at the end of the experiment (IgG dEnd). The curves represent the mean IgG response from four mice in each group. b, e Tick weights after feeding on OVA- and tHRF-immunized mice. c, f Percentage of fully engorged ticks recovered throughout the experiment. The mice were immunized using aluminum hydroxide (a–c) and Freundʼs adjuvant (d–f)