Table 2 Primers used in this study
Experiment | Description | Sequence (5′–3′) |
|---|---|---|
Real-time PCR | HlP0-Fwd | CTCCATTGTCAACGGTCTCA |
HlP0-Rev | TCAGCCTCCTTGAAGGTGAT | |
HlVgR-Fwd | GACCGTTCTTGCTTCTGTCC | |
HlVgR-Rev | GCGACCATCACACCAGTAGA | |
In situ hybridization | T7-HlVgR-1-Fwd | TAATACGACTCACTATAGGCTGTCCCTCGGATGACTTCTCCTG |
HlVgR-1-Rev | CCCCACAGTCGATCATGCCA | |
HlVgR-1-Fwd | CTGTCCCTCGGATGACTTCTCCTG | |
SP6-HlVgR-1-Rev | ATTTAGGTGACACTATAGCCCCACAGTCGATCATGCC | |
Expression of recombinant HlVgR protein | XhoI-HlVgR-Fwd | CCGCTCGAGATCTACTGGTGTGATGGT |
EcoRI-HlVgR-Rev | GGAATTCACAGTGGCTGCTTTCGT |