Role of gamma-giardin in ventral disc formation of Giardia lamblia

Parasites & Vectors

Table 3 Identification proteins by 2D analysis

	GiardiaDB ID	Protein name^a	MW (kDa)^a	pI^a	Sequence coverage (%) ^b	Matched peptide number^b	Expected value^c
1	GL50803_88765	Cytosolic HSP70	72	5.26	36	20	7.6e−014
2	GL50803_17063	Pyruvate-flavodoxin oxidoreductase	133	6.41	22	25	1.9e−011
3	GL50803_10521	Arginyl-tRNA synthetase	71	6.48	23	14	0.0022
4	GL50803_9008	Aldose reductase	35	6.61	29	9	0.0022
5	GL50803_16717	Hypothetical protein	29	6.54	44	11	1.2e−005
6	GL50803_11118	Enolase	49	5.46	23	9	0.052
7	GL50803_137703	Protein 21.1	57	5.97	28	15	0.0071
8	GL50803_16124	TCP-1 chaperonin subunit eta	66	7.5	26	15	0.047
9	GL50803_14404	Phosphatase	43	6.14	34	10	0.00039
10	GL50803_21942	NADP-specific glutamate dehydrogenase	50	7.98	51	26	4.8e−018
11	GL50803_16453	Carbamate kinase	34	8.03	44	16	3e−009
12	GL50803_103713	Protein disulfide isomerase 4	41	6.13	35	11	0.00024
13	GL50803_103373	alpha-7.1 giardin	44	8.81	28	12	0.0001
14	GL50803_11043	Fructose-bisphosphate aldolase	35	6.19	34	9	0.028
15	GL50803_17230	Gamma giardin	36	6.48	50	21	0.003
16	GL50803_13273	Dynein light intermediate chain	27	7.68	35	11	0.0012
17	GL50803_17090	Giardia trophozoite antigen 1	21	5.05	56	18	1.9e−011
18	GL50803_5333	Calmodulin	17	4.29	66	7	0.00048
19	GL50803_6171	Hypothetical protein	17	9.12	47	8	0.059

^aData from 2D gel
^bMatching masses from LC–MS/MS when searching against all entries in NCBI using Mascot search engine
^cProbability value obtained from Mascot search

ISSN: 1756-3305