Skip to main content

Advertisement

Table 3 Identification proteins by 2D analysis

From: Role of gamma-giardin in ventral disc formation of Giardia lamblia

  GiardiaDB ID Protein namea MW (kDa)a pIa Sequence coverage (%) b Matched peptide numberb Expected valuec
1 GL50803_88765 Cytosolic HSP70 72 5.26 36 20 7.6e−014
2 GL50803_17063 Pyruvate-flavodoxin oxidoreductase 133 6.41 22 25 1.9e−011
3 GL50803_10521 Arginyl-tRNA synthetase 71 6.48 23 14 0.0022
4 GL50803_9008 Aldose reductase 35 6.61 29 9 0.0022
5 GL50803_16717 Hypothetical protein 29 6.54 44 11 1.2e−005
6 GL50803_11118 Enolase 49 5.46 23 9 0.052
7 GL50803_137703 Protein 21.1 57 5.97 28 15 0.0071
8 GL50803_16124 TCP-1 chaperonin subunit eta 66 7.5 26 15 0.047
9 GL50803_14404 Phosphatase 43 6.14 34 10 0.00039
10 GL50803_21942 NADP-specific glutamate dehydrogenase 50 7.98 51 26 4.8e−018
11 GL50803_16453 Carbamate kinase 34 8.03 44 16 3e−009
12 GL50803_103713 Protein disulfide isomerase 4 41 6.13 35 11 0.00024
13 GL50803_103373 alpha-7.1 giardin 44 8.81 28 12 0.0001
14 GL50803_11043 Fructose-bisphosphate aldolase 35 6.19 34 9 0.028
15 GL50803_17230 Gamma giardin 36 6.48 50 21 0.003
16 GL50803_13273 Dynein light intermediate chain 27 7.68 35 11 0.0012
17 GL50803_17090 Giardia trophozoite antigen 1 21 5.05 56 18 1.9e−011
18 GL50803_5333 Calmodulin 17 4.29 66 7 0.00048
19 GL50803_6171 Hypothetical protein 17 9.12 47 8 0.059
  1. aData from 2D gel
  2. bMatching masses from LC–MS/MS when searching against all entries in NCBI using Mascot search engine
  3. cProbability value obtained from Mascot search