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Fig. 9 | Parasites & Vectors

Fig. 9

From: Delivery of SA35 and SA40 peptides in mice enhances humoral and cellular immune responses and confers protection against Cryptosporidium parvum infection

Fig. 9

Quantification of COWP gene DNA copies by qPCR in the intestinal content of neonate mice infected with 5 × 103 Cryptosporidium parvum oocysts. Values are expressed as the mean of cycles for crossing thresholds (CT), and each sample was tested in triplicate. Standard curve (black dots) was obtained from DNA extracted from the following oocysts dilutions: 1 × 105 (1), 1 × 104 (2), 1 × 103 (3), 100 (4), 10 (5), 1 (6) and 0.1 (7). Blue dots represent CT values from control mice. Red dots represent CT values from immunised mice. Values from control mice are between 33 and 29 cycles, equivalent to DNA amounts from 6 to 100 oocysts, whereas values from immunised mice are between 39 to 35 cycles, equivalent to DNA amounts from 0.1 to 5 oocysts

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