Delivery of SA35 and SA40 peptides in mice enhances humoral and cellular immune responses and confers protection against Cryptosporidium parvum infection

Table 1 Interferon (IFN)-γ, interleukin (IL)-4 and IL-12-40p, produced by splenocytes from immunised mice

Immunised group	Stimulant	IFN γ		IL-4		IL-12 40p
Immunised group	Stimulant	Mean, pg/ml (range)	No. of mice^a	Mean, pg/ml (range)	No. of mice^a	Mean, pg/ml (range)	No. of mice^a
SA 35	None	< 15 (< 15)	0/5	11 (< 4–27.13)	2/5	197 (24–436)	5/5
	CCE	81 (15–350)	2/5	23 (< 4–93.5)	2/5	274 (14–738)	4/5
	SA 35	1082 (15–1623)	5/5	59.3 (< 4–162)	3/5	522 (67–960)	5/5
SA 40	None	< 15 (< 15)	0/4	10 (< 4–16)	2/4	84 (19–260)	4/4
	CCE	45 (15–58)	4/4	7 (< 4–17)	1/4	44 (13–93)	3/4
	SA 40	692 (15–1605)	4/4	20 (< 4–52)	2/4	84 (18–202)	4/4
SA35-40 mix	None	< 15 (< 15)	0/5	20 (< 4–20)	1/5	25 (18–45)	5/5
	CCE	30 (15–80)	4/5	88 (< 4–88)	1/5	12 (4–18)	4/5
	SA 35/40 mix	26 (15–86)	5/5	34 (< 4–79)	3/5	62 (12–140)	4/5

Notes: Mice were immunised with SA35, SA40 peptides or SA35-40 mix. Splenocytes were cultured in absence of stimulating factor (None), or stimulated with C. parvum crude extract (CCE) or the same antigen preparation used for the immunisation (i.e. SA35 or SA40 or SA35/40 mix). Cytokines were assayed on the culture supernatant. Concentrations ≥ 15 pg/ml for IFN-γ, ≥ 4 pg/ml for IL-4 and ≥ 14 pg/ml for > IL-12 40p were considered positives
^aNo. positive/no. examined

ISSN: 1756-3305