|
Immunised group
|
Stimulant
|
IFN γ
|
IL-4
|
IL-12 40p
|
|---|
|
Mean, pg/ml (range)
|
No. of micea
|
Mean, pg/ml (range)
|
No. of micea
|
Mean, pg/ml (range)
|
No. of micea
|
|---|
|
SA 35
|
None
|
< 15 (< 15)
|
0/5
|
11 (< 4–27.13)
|
2/5
|
197 (24–436)
|
5/5
|
|
CCE
|
81 (15–350)
|
2/5
|
23 (< 4–93.5)
|
2/5
|
274 (14–738)
|
4/5
|
|
SA 35
|
1082 (15–1623)
|
5/5
|
59.3 (< 4–162)
|
3/5
|
522 (67–960)
|
5/5
|
|
SA 40
|
None
|
< 15 (< 15)
|
0/4
|
10 (< 4–16)
|
2/4
|
84 (19–260)
|
4/4
|
|
CCE
|
45 (15–58)
|
4/4
|
7 (< 4–17)
|
1/4
|
44 (13–93)
|
3/4
|
|
SA 40
|
692 (15–1605)
|
4/4
|
20 (< 4–52)
|
2/4
|
84 (18–202)
|
4/4
|
|
SA35-40 mix
|
None
|
< 15 (< 15)
|
0/5
|
20 (< 4–20)
|
1/5
|
25 (18–45)
|
5/5
|
|
CCE
|
30 (15–80)
|
4/5
|
88 (< 4–88)
|
1/5
|
12 (4–18)
|
4/5
|
|
SA 35/40 mix
|
26 (15–86)
|
5/5
|
34 (< 4–79)
|
3/5
|
62 (12–140)
|
4/5
|
- Notes: Mice were immunised with SA35, SA40 peptides or SA35-40 mix. Splenocytes were cultured in absence of stimulating factor (None), or stimulated with C. parvum crude extract (CCE) or the same antigen preparation used for the immunisation (i.e. SA35 or SA40 or SA35/40 mix). Cytokines were assayed on the culture supernatant. Concentrations ≥ 15 pg/ml for IFN-γ, ≥ 4 pg/ml for IL-4 and ≥ 14 pg/ml for > IL-12 40p were considered positives
- aNo. positive/no. examined
