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Table 1 Interferon (IFN)-γ, interleukin (IL)-4 and IL-12-40p, produced by splenocytes from immunised mice

From: Delivery of SA35 and SA40 peptides in mice enhances humoral and cellular immune responses and confers protection against Cryptosporidium parvum infection

Immunised group Stimulant IFN γ IL-4 IL-12 40p
Mean, pg/ml (range) No. of micea Mean, pg/ml (range) No. of micea Mean, pg/ml (range) No. of micea
SA 35 None < 15 (< 15) 0/5 11 (< 4–27.13) 2/5 197 (24–436) 5/5
CCE 81 (15–350) 2/5 23 (< 4–93.5) 2/5 274 (14–738) 4/5
SA 35 1082 (15–1623) 5/5 59.3 (< 4–162) 3/5 522 (67–960) 5/5
SA 40 None < 15 (< 15) 0/4 10 (< 4–16) 2/4 84 (19–260) 4/4
CCE 45 (15–58) 4/4 7 (< 4–17) 1/4 44 (13–93) 3/4
SA 40 692 (15–1605) 4/4 20 (< 4–52) 2/4 84 (18–202) 4/4
SA35-40 mix None < 15 (< 15) 0/5 20 (< 4–20) 1/5 25 (18–45) 5/5
CCE 30 (15–80) 4/5 88 (< 4–88) 1/5 12 (4–18) 4/5
SA 35/40 mix 26 (15–86) 5/5 34 (< 4–79) 3/5 62 (12–140) 4/5
  1. Notes: Mice were immunised with SA35, SA40 peptides or SA35-40 mix. Splenocytes were cultured in absence of stimulating factor (None), or stimulated with C. parvum crude extract (CCE) or the same antigen preparation used for the immunisation (i.e. SA35 or SA40 or SA35/40 mix). Cytokines were assayed on the culture supernatant. Concentrations ≥ 15 pg/ml for IFN-γ, ≥ 4 pg/ml for IL-4 and ≥ 14 pg/ml for > IL-12 40p were considered positives
  2. aNo. positive/no. examined