Fig. 1

Aedes aegypti SGE inhibits NO production and iNOS expression by M1-polarized murine macrophages. Thioglycolate-elicited peritoneal macrophages were collected and cultured as described in “Methods”. Cells were preincubated with complete medium (control group) or with SGE (final concentration indicated in the figure; otherwise 40 µg/ml) for 1 h and stimulated or not with LPS plus IFN-γ (final concentration: 10 ng/ml of each). NO production was indirectly estimated after 48 h in culture supernatants by Griess reaction (a). iNOS expression was evaluated after 24 h in cell lysates by Western blot (b). The relative expression of iNOS was determined by densitometry and the results were presented as percentage in relation to the control group (considered as 100%) (c). Results are expressed as the mean ± SEM. *P < 0.05 versus control group (cells incubated with medium only); ^#P < 0.05 versus “LPS + IFN-γ” group