Fig. 2

Aedes aegypti SGE affects viability of murine lymphocytes but not of macrophages. Thioglycolate-elicited peritoneal macrophages and total spleen cells were collected and cultured as described in “Methods”. Cells were maintained into culture tubes and incubated with complete medium (control group) or with SGE (final concentration: 10 and 40 µg/ml) for 4 h. Annexin V staining was evaluated by flow cytometry in CD3⁺ cells (T lymphocytes) (a), CD19⁺ cells (B lymphocytes) (b), and CD11b⁺F4/80⁺ cells (macrophages) (c). In another set of experiments, cells were preincubated with complete medium (control group) or with SGE (final concentration: 10 and 40 µg/ml) for 1 h and stimulated with Con A (0.5 μg/ml final concentration) for spleen cell cultures (d) or LPS plus IFN-γ (10 ng/ml of each, final concentration) for macrophage cultures (e). Twenty-five microliters of 0.01% resazurin were added to the cultures and after 48 h, cell viability was evaluated by reading the cultures absorbance at 570 and 600 nm. Results are expressed as the mean ± SEM. *P < 0.05 versus control group (cells incubated with medium only); ^#P < 0.05 versus “SGE 10” group