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Fig. 5 | Parasites & Vectors

Fig. 5

From: Aedes aegypti saliva impairs M1-associated proinflammatory phenotype without promoting or affecting M2 polarization of murine macrophages

Fig. 5

Aedes aegypti SGE does not bind murine IL-6 or IL-12. Different concentrations of Ae. aegypti SGE (1, 5 and 10 µg/ml) were preincubated with a serial dilution of mouse recombinant IL-6 or IL-12 for 15 minutes at 37 °C. Samples were then transferred to ELISA plates coated with anti-IL-6 or anti-IL-12 capture monoclonal antibody. For each case, a control group (a serial dilution of recombinant cytokine diluted in buffer only) was assayed under the same conditions (a, c). ELISA plate wells were coated with Ae. aegypti SGE (10 µg/ml), anti-IL-6 or anti-IL-12 antibody and incubated with serial dilutions of recombinant IL-6 or IL-12 (b, d). The detection of the cytokines was performed as described in “Methods”. Results are expressed as the mean ± SEM

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