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Fig. 6 | Parasites & Vectors

Fig. 6

From: Aedes aegypti saliva impairs M1-associated proinflammatory phenotype without promoting or affecting M2 polarization of murine macrophages

Fig. 6

Aedes aegypti SGE impairs M1 but not M2 polarization. Thioglycolate-elicited peritoneal macrophages were collected and cultured as described in “Methods”. Cells were preincubated with complete medium (control group) or with SGE (final concentration: 40 µg/ml) for 1 h and maintained in medium (M0 condition), stimulated with LPS plus IFN-γ (final concentration: 10 ng/ml of each) for M1 polarization or with IL-4 (final concentration: 20 ng/ml) for M2 polarization. After 48 h, culture supernatants were collected for NO determination by Griess reaction (a) and the cell lysate were prepared for urea determination as a product of the arginase activity (b). Arginase-1 (c) and mannose receptor-1 (MRC-1) (e) expression were evaluated by Western blot. The relative expression of arginase-1 and MRC-1 were determined by densitometry and the results were presented as percentage in relation to the control group (considered as 100%) (d and f, respectively). Results are expressed as the mean ± SEM. *P < 0.05 versus respective control group (cells incubated with medium or SGE only)

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