Table 1 Primers used in the ETAS-PCR/Vgsc-1014, pyrosequencing and TaqMan assays for genotyping the Vgsc-L1014F in C. quinquefasciatus mosquitoes. The Eco32I site is underlined
Primer | Sequence (5′–3′) | Fragment size (bp) |
|---|---|---|
Vgsc-1014/F-T | AGTAGCGGATAACAATTTCACACAGGAAGGGTTTTCCCAGTCACGACGTTGATATCGCCACCGTAGTGATAGGAAATATT | 181 |
Vgsc-1014/F-A | AGTAGCGGATAACAATTTCACACAGGATATCGAAGGGTTTTCCCAGTCACGACGTTGCCACCGTAGTGATAGGAAATTCA | 206 |
Vgsc-1014/F-C | GATATCAGTAGCGGATAACAATTTCACACAGGAAGGGTTTTCCCAGTCACGACGTTGCCACCGTAGTGATAGGAAACTTC | 231 |
Vgsc-1014/R | GATCGGTATGAACTGTTTGTTTACATC | |
Pyrosequencing assay | ||
pyr-Vgsc-F | CTTGGCCACCGTAGTGATAGG | 105 |
pyr-Vgsc-R | Biotin-GCTGTTGGCGATGTTTTGACA | |
pyrSeq. primer | CCGTAGTGATAGGAAATTT | |
Dispensation | (A/C/T)GTCGTGAGTATTCCAGCGTGAAGTC | |
TaqMan assay | ||
TaqMan-F | CTTGGCCACCGTAGTGATAGG | |
TaqMan-R | GCTGTTGGCGATGTTTTGACA | |
TaqMan-Probe-TTC | FAM-CACGACGAAATTT | |
TaqManProbe-TTT | FAM-TCACGACAAAATTT | |
TaqManProbe-TTA | VIC-ACTCACGACTAAATTT | |