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Table 2 Specificity of ETAS-PCR Vgsc-1014 in comparison to pyrosquencing and TaqMan genotyping

From: Development and application of a tri-allelic PCR assay for screening Vgsc-L1014F kdr mutations associated with pyrethroid and organochlorine resistance in the mosquito Culex quinquefasciatus

Country Brazil Uganda Tanzania
Location Campina Grande Tororoa Tororo Jinja Mwanza
Genotype ETAS-PCR TaqMan ETAS-PCR TaqMan ETAS-PCR Pyr ETAS-PCR Pyr ETAS-PCR Pyr
TTA/TTA 24 24 23 23 2 1 4 4 3 3
TTC/TTC    15 15 10 10 4 4 2 2
TTT/TTT    1 1    1 1 1 1
TTA/TTC    23 23 1 1 9 9 8 8
TTA/TTT    9 9    3 3 7 7
TTC/TTT    13 13 12 13 3 3 2 2
N (98)   24   84   28   24   23
  1. aMosquito exposed to insecticides (WHO—Tube Bioassay)
  2. Pyr; Pyrosequencing