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Table 2 Specificity of ETAS-PCR Vgsc-1014 in comparison to pyrosquencing and TaqMan genotyping

From: Development and application of a tri-allelic PCR assay for screening Vgsc-L1014F kdr mutations associated with pyrethroid and organochlorine resistance in the mosquito Culex quinquefasciatus

Country

Brazil

Uganda

Tanzania

Location

Campina Grande

Tororoa

Tororo

Jinja

Mwanza

Genotype

ETAS-PCR

TaqMan

ETAS-PCR

TaqMan

ETAS-PCR

Pyr

ETAS-PCR

Pyr

ETAS-PCR

Pyr

TTA/TTA

24

24

23

23

2

1

4

4

3

3

TTC/TTC

  

15

15

10

10

4

4

2

2

TTT/TTT

  

1

1

  

1

1

1

1

TTA/TTC

  

23

23

1

1

9

9

8

8

TTA/TTT

  

9

9

  

3

3

7

7

TTC/TTT

  

13

13

12

13

3

3

2

2

N (98)

 

24

 

84

 

28

 

24

 

23

  1. aMosquito exposed to insecticides (WHO—Tube Bioassay)
  2. Pyr; Pyrosequencing