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Fig. 1 | Parasites & Vectors

Fig. 1

From: Aquaporin 1 is located on the intestinal basolateral membrane in Toxocara canis and might play a role in drug uptake

Fig. 1

Expression, purification and western blotting of recombinant TcAQP1c peptide. a SDS-PAGE analysis of the prokaryotic expression of recombinant TcAQP1c. Lane M: protein molecular weight maker; Lane 1: supernatant expression of induced empty pET32a; Lane 2: insoluble expression of induced empty pET32a; Lane 3: supernatant expression of non-induced recombinant pET32a plasmids; Lane 4: insoluble expression of non-induced recombinant pET32a plasmids; Lane 5: supernatant expression of induced recombinant pET32a plasmids; Lane 6: insoluble expression of induced recombinant pET32a plasmids. b Purification of recombinant TcAQP1c peptide. Lane M: protein molecular weight maker; Lanes 1–5: elution using 20 mM, 50 mM, 100 mM, 250 mM or 500 mM of imidazole, respectively. c Western blot analysis of recombinant TcAQP1c peptide. Lane M: protein molecular weight maker; Lane 1: polyclonal antibody against the recombinant TcAQP1c peptide; Lane 2: pre-immune serum

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