Table 1 JEV seropositivity among donor birds, wild boars and sentinel chickens. Seropositivity was confirmed by PRNT after IFA screening. PRNT80 end point titre was determined by counting plaques and reporting the titre as the reciprocal of the last serum dilution to show 80% reduction of control plaques (≤ 20 plaques) counted based on the 100 pfu of virus added to each sera sample
Sample ID | Bird species | Sex/Age group | Location | Date collected | PRNT80 (end-point titer) |
|---|---|---|---|---|---|
DB2 | Crested goshawk (Accipiter trivirgatus) | na | Bukit Panjang | March 2011 | 103 |
DB3 | White-bellied sea eagle (Haliaeetus leucogaster) | na | Changi Airport | March 2011 | 102 |
DB4 | Changeable hawk-eagle (Nisaetus cirrhatus) | na | Jurong West HDB | April 2011 | 102 |
DB9 | Crested serpent eagle (Spilornis cheela) | na | Not available | May 2011 | 103 |
DB13 | Oriental honey-buzzard (Pernis ptilorhyncus) | na | Japanese Garden | May 2011 | 102 |
DB14 | Black-crowned night heron (Nycticorax nycticorax) | na | Pasir Ris Park | May 2011 | 101 |
DB20 | White-bellied sea eagle (Haliaeetus leucogaster) | na | Lim Chu Kang MRT | Not available | 102 |
DB31 | White-bellied sea eagle (Haliaeetus leucogaster) | na | Upper Seletar Reserve | April 2011 | 101 |
DB38 | White-bellied sea eagle (Haliaeetus leucogaster) | na | Tuas | March 2012 | 101 |
DB41 | Cattle egret (Bubulcus ibis) | na | Jurong East | July 2012 | 103 |
DB59 | Little egret (Egretta garzetta) | na | Japanese Garden | October 2012 | 101 |
DB71 | Heron (not identified to species) | na | Avian Sanctuary | Not available | 102 |
MP10 | Wild boar (Sus scrofa) | na | Central Catchment Nature Reserve | Mar 2013 | 102 |
MP12 | Wild boar (Sus scrofa) | na | Central Catchment Nature Reserve | April 2013 | 101 |
MP19 | Wild boar (Sus scrofa) | na | Central Catchment Nature Reserve | June 2013 | 102 |
MP27 | Wild boar (Sus scrofa) | na | Central Catchment Nature Reserve | July 2013 | 101 |
MP32 | Wild boar (Sus scrofa) | Male; juvenile | Central Catchment Nature Reserve | September 2013 | 101 |
MP33 | Wild boar (Sus scrofa) | Male; adult | Central Catchment Nature Reserve | December 2013 | 101 |
MP38 | Wild boar (Sus scrofa) | Male; juvenile | Central Catchment Nature Reserve | December 2013 | 102 |
MP41 | Wild boar (Sus scrofa) | Male; juvenile | Central Catchment Nature Reserve | December 2013 | 102 |
MP44 | Wild boar (Sus scrofa) | Female; adult | Central Catchment Nature Reserve | January 2014 | 102 |
MP45 | Wild boar (Sus scrofa) | Female; piglet | Central Catchment Nature Reserve | January 2014 | 103 |
SC281013Gr | Chicken (Gallus domesticus) | Female; adult | Nature Reserve | October 2013 | 101 |
SC281013Pu | Chicken (Gallus domesticus) | Female; adult | Nature Reserve | October 2013 | 101 |
SC281013Ye | Chicken (Gallus domesticus) | Female; adult | Nature Reserve | October 2013 | 102 |
SC031114Bl | Chicken (Gallus domesticus) | Female; adult | Nature Reserve | November 2014 | 101 |
SC031114Re | Chicken (Gallus domesticus) | Female; adult | Nature Reserve | November 2014 | 101 |
SC031114Pu | Chicken (Gallus domesticus) | Female; adult | Nature Reserve | November 2014 | 102 |