Fig. 3

Simulated-field and semi-field evaluation of sema.460 activity in Ae. aegypti. a Activity of sema.460 was confirmed in trials conducted in rainwater. Data shown were compiled from two biological replicate experiments and represent results from six container assays, each with 20 larvae for a total of 120 larvae assessed/condition. b Activity was also confirmed in F2 larvae from a Trinidad field strain. Data were compiled from three replicate experiments conducted on a total of 240 larvae/condition that were assessed in 12 containers, each with 20 larvae. c The activity of sema.460 was also confirmed in semi-field trials. Data were compiled from three biological replicate experiments conducted on a total of 180 animals/treatment in nine replicate containers with 20 larvae each. d Semi-field trials were conducted in a contained outdoor roof top laboratory. Mean percentages of larval mortality are shown in a–c. Data in a–c were analyzed with t-tests; ***P < 0.001 when compared with control-fed larvae