Fig. 5

sema.460 is not toxic to three non-target arthropods. a Oral consumption of sema.460 by D. melanogaster larvae did not impact survival through adult emergence (a total of 20 larvae/treatment were assessed in two biological replicate experiments, each conducted with 10 larvae/treatment). b Consumption of sema.460 by D. pulex did not impact adult survival (30 animals/treatment were evaluated in three biological replicate experiments in which 10 animals/treatment were assessed over a 10-day trial period). c Likewise, sema.460 was not toxic to D. magna (30 animals/treatment were evaluated in three biological replicate experiments in which 10 animals/treatment were assessed for a 10-day trial period). Control groups in all of these assays were treated with yeast prepared from yeast expressing control shRNA. Survival data compiled from two (a) or three (b, c) replicate experiments and analyzed with the Fisher’s exact test indicated that there were no significant differences in the survival of sema.460- and control-treated organisms. Data shown represent mean percentages of survival