Fig. 3

Gating of leukocyte subpopulations by flow cytometry. The figure shows forward versus sideward scatter plots of caudal fin (a–d), spleen (e–h), head kidney (i–l) tissues. The small leukocytes with low granularity are located FSC^low; SSC^low and are identified as lymphocytes. The bigger cells with a higher granularity are found FSC^high; SSC^high and recognized as myeloid cells