Fig. 4

Growth inhibition of EA, β-CD EA and APSP EA on B. microti in vivo. Graph showing the inhibitory effects of DA-IP, EA-IP, β-CD EA-IP, β-CD EA-oral, APSP EA-IP and APSP EA-oral treatment compared to the untreated group. The values plotted indicate the mean ± standard deviation for two separate experiments. Asterisks (*) indicate statistically significant differences (P < 0.05) based on the unpaired t-test analysis. The arrow indicates 5 consecutive days of treatment. Parasitemia was calculated by counting infected RBCs among 5000 RBCs using Giemsa-stained thin blood smears. Abbreviations: DA, diminazene aceturate; EA, ellagic acid; β-CD EA, β-cyclodextrin ellagic acid; APSP EA, antisolvent precipitation with syringe pump prepared ellagic acid; IP, intraperitoneal; RBCs, red blood cells