Fig. 5

Growth inhibition of APSP-ellagic acid on B. microti in vivo. Graph showing the inhibitory effects of DA-IP, AQ-oral, CF-oral, APSP EA-IP, APSP EA-DA, APSP EA-AQ and APSP EA-CF treatment as compared to the untreated group. The values plotted indicate the mean ± standard deviation for two separate experiments. Asterisks (*) indicate statistically significant differences (P < 0.05) based on the unpaired t-test analysis. The arrow indicates 5 consecutive days of treatment. Parasitemia was calculated by counting infected RBCs among 5000 RBCs using Giemsa-stained thin blood smears. Abbreviations: DA, diminazene aceturate; AQ, atovaquone; CF, clofazimine; EA, ellagic acid; APSP EA, antisolvent precipitation with syringe pump prepared ellagic acid; IP, intraperitoneal; RBCs, red blood cells