Fig. 3

The overexpression of LmjF.22.0600 increases curcumin- and miltefosine-induced cell death. a Cell concentration of WT cells and cells overexpressing LmjF.22.0600 [WT(LmjF.22.0600)] in untreated cells and cells treated with curcumin (50 µM), miltefosine (40 µM) and pentamidine (50 µM), as measured with a hemocytometer. b IC50 values of curcumin, miltefosine and pentamidine in the WT strain and the strain overexpressing LmjF.22.0600, from MTT assays. c Percentage of dying cells, i.e. TUNEL-positive cells and cells without a nucleus, in the WT and LmjF.22.0600 overexpressing strains, when left untreated or treated with 50 µM curcumin or 40 µM miltefosine for 24 h. Values are shown as the mean ± SD. The number of independent experiments that were carried out is given below the bars. Unpaired Wilcoxon-Mann-Whitney test: ns, not significant, *P < 0.05 and **P < 0.01