Fig. 5From: Cobalt nanoparticles as novel nanotherapeutics against Acanthamoeba castellaniiExcystation assay was performed to evaluate the differentiation of A. castellanii from cysts into trophozoites. Briefly, 5 × 105 A. castellanii cysts were incubated with CoNPs in growth medium (PYG) at 30 °C for 72 h. After incubation, cysts emerged into trophozoites were enumerated using hemocytometer. The results show significant inhibition of encystation when compared to the control. *P < 0.05Back to article page