Fig. 7From: Cobalt nanoparticles as novel nanotherapeutics against Acanthamoeba castellaniiPre-treatment of amoeba with CoNPs inhibited HeLa cell cytopathogenicity. In brief, 5 × 105 A. castellanii trophozoites were incubated with 100 µg/ml of CoNPs at 30 °C for 2 h. Treated amoeba were then added to HeLa cells and incubated for 24 h at 37 °C in a 5% CO2 incubator. The results show significant inhibition of cytopathogenicity when compared to untreated amoeba. *P < 0.05Back to article page