Fig. 2

Effects of T. gondii infection on ATP-induced apoptosis of mouse RAW264.7 macrophage cells. RAW264.7 cells were infected with the RH, ME49 or VEG strain of T. gondii and followed by ATP induction 4 or 6 h. The cells were harvested at 6 or 28 h post-infection for apoptosis measurement by flow cytometry after annexin V-FITC/PI staining. Representative flow cytometry data are presented in panel a and quantified in panels b1 and b2. The experiments were repeated four times. The values were analyzed using the Kruskal–Wallis H-test and Bonferroni correction (*P < 0.05, **P < 0.01)