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Fig. 6 | Parasites & Vectors

Fig. 6

From: Toxoplasma gondii ROP18 inhibits human glioblastoma cell apoptosis through a mitochondrial pathway by targeting host cell P2X1

Fig. 6

TgROP18 inhibition of host cell apoptosis promoted by P2X1. Four groups of SF268 cells were treated or not with ATP and/or NF449 as indicated. Five groups of COS7 cells were prepared. The N group is the normal COS7 cells. The other four groups were transfected with pcDNA3.1-ROP18 and/or pcDNA3.1-P2X1 and followed by treatment of ATP and/or NF449 as indicated. a Representative flow cytometry data. The apoptosis rate was measured after annexin V-FITC/PI staining. b Western blotting analysis of the expression of Flag-tagged ROP18 and HA-tagged P2X1 in COS7 cells. c, d Quantification of the flow cytometry data. The flow cytometry experiments were repeated four times. The values were analyzed using the Kruskal–Wallis H-test and Bonferroni correction (*P < 0.05, **P < 0.01)

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