Fig. 7
TgROP18 inhibition of P2X1-mediated calcium influx. a Expression of Flag-tagged ROP18 and HA-tagged P2X1. COS7 cells were transfected with pcDNA3.1-P2X1-HA and/or pcDNA3.1-ROP18-3×Flag. Production of the ROP18 and P2X1 proteins was determined by western blotting. b Calcium influx measurement. The transfected cells in A were stimulated with ATP, and Ca2+ influx in each group was analyzed using flow cytometry. The maximum elevation (F/F0) of the intracellular Ca2+ were analyzed using the Kruskal–Wallis H-test and Bonferroni correction (*P < 0.05). c P2X1 protein integrity. COS7 cells were transfected with a stable amount (2.0 µg) of pcDNA3.1-P2X1-HA and sequentially co-transfected with 0, 0.5, 1.0 and 2.0 µg of pcDNA3.1-ROP18-3×Flag. The integrity of the proteins was analyzed by western blotting