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Fig. 2 | Parasites & Vectors

Fig. 2

From: Role of the lipoxin A4 receptor in the development of neutrophil extracellular traps in Leishmania infantum infection

Fig. 2

Flow cytometry analysis of H3Cit formation in neutrophils from whole blood stimulated by L. infantum promastigotes for 5 h. Whole blood pre-treated with or without the antagonist (Boc) or the agonist lipoxin A4 (LXA4) were exposed to L. infantum promastigotes or left untreated for 5 h at 37 °C. Percentage of H3Cit formation in neutrophils was quantified by flow cytometric assay. a Percentage of H3Cit-positive neutrophils in experimental group Neu; b Percentage of H3Cit-positive neutrophils in experimental group Neu+Boc+Leish; c Percentage of H3Cit-positive neutrophils in experimental group Neu+Leish; d Isotype of H3Cit; e Percentage of H3Cit-positive neutrophils in experimental group Neu+LXA4; f Percentage of H3Cit-positive neutrophils in experimental group Neu+LXA4+Leish; g Percentage of H3Cit-positive neutrophils in experimental group Neu+Boc+LXA4; h Statistical analysis of the H3Cit-positive neutrophil percentages among experimental groups. The differences were analyzed using one-way ANOVA, then LSD was applied in the following two groups’ comparison. *P < 0.05, **P < 0.01, ***P < 0.001. Abbreviations: Neu: control group of untreated whole blood; Neu+Boc+Leish, Boc-pretreated whole blood stimulated by L. infantum promastigotes; Neu+Leish, untreated whole blood stimulated by L. infantum promastigotes; Neu+LXA4, untreated neutrophils stimulated by LXA4; Neu+LXA4+Leish, LXA4-pretreated neutrophils stimulated by promastigotes; Neu+Boc+LXA4, Boc-pretreated neutrophils stimulated by LXA4

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