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Fig. 1 | Parasites & Vectors

Fig. 1

From: Molecular probes for the identification of avian Haemoproteus and Leucocytozoon parasites in tissue sections by chromogenic in situ hybridization

Fig. 1

Erythrocytic and exo-erythrocytic parasite stages in lung sections from infected birds visualized by chromogenic in situ hybridization (CISH). Serial lung sections from a yellowhammer (Emberiza citrinella) infected with Haemoproteus sp. lineage EMCIR01 (a, b) and a crow (Corvus cornix) infected with Leucocytozoon sp., lineage COCOR13 (c, d) were stained with haematoxylin–eosin (HE; a, c) and subjected to CISH (b, d). Gametocytes (arrowheads) and exo-erythrocytic meronts (arrows) of parasites were observed in HE-stained sections (a, c) and labelled by subgenus-specific probes for Haemoproteus (Parahaemoproteus) spp. (b) and Leucocytozoon (Leucocytozoon) spp. (d) showing distinct purple signals in corresponding in situ-hybridized sections. Note that small parasites are difficult to see in HE-stained sections, but are readily visible in sections subjected to CISH. Scale-bars: 20 µm (inserts, 10 µm)

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