Table 1 Oligonucleotide sequences of primers and probes used for real-time PCRs and conventional PCRs in this study
Target | Name | Sequence (5′–3′) and probes | Source |
|---|---|---|---|
P. humanus Cytochrome b | Duplex A–D | F: GATGTAAATAGAGGGTGGTT | |
R: GAAATTCCTGAAAATCAAAC | |||
FAM-CATTCTTGTCTACGTTCATATTTGG-TAMRA | |||
VIC-TATTCTTGTCTACGTTCATGTTTGA-TAMRA | |||
Duplex B–C/E | F: TTAGAGCGMTTRTTTACCC | ||
R: AYAAACACACAAAAMCTCCT | |||
FAM-GAGCTGGATAGTGATAAGGTTTAT-MGB | |||
VIC-CTTGCCGTTTATTTTGTTGGGGTTT-TAMRA | |||
Monoplex E | F: GGTTGGAATTGGATAGTGAT | ||
R: GGGTCCATAAAGAAATCCG | |||
FAM- TAGGAGGCTTTGTGTGTCTATCCT-TAMRA | |||
Cytb | F: GAGCGACTGTAATTACTAATC | [24] | |
R: CAACAAAATTATCCGGGTCC | |||
Acinetobacter spp. RNA polymerase β subunit gene | rpoB | F: TACTCATATACCGAAAAGAAACGG | [18] |
R: GGYTTACCAAGRCTATACTCAAC | |||
FAM-CGCGAAGATATCGGTCTSCAAGC-TAMRA | |||
rpoB (zone1) | F: TAYCGYAAAGAYTTGAAAGAAG | [25] | |
R: CMACACCYTTGTTMCCRTGA | |||
R. prowazekii rOmpB gene | ompB | F: AATGCTCTTGCAGCTGGTTCT | |
R: TCGAGTGCTAATATTTTTGAAGCA | |||
FAM-CGGTGGTGTTAATGCTGCGTTACAACA-TAMRA | |||
Y. pestis Plasminogen activator gene | PLA | F: ATGGAGCTTATACCGGAAAC | [26] |
R: GCGATACTGGCCTGCAAG | |||
FAM-TCCCGAAAGGAGTGCGGGTAATAGG-TAMRA | |||
Borrelia spp. 16S ribosomal RNA | Bor16S | F: AGCCTTTAAAGCTTCGCTTGTAG | [27] |
R: GCCTCCCGTAGGAGTCTGG | |||
FAM-CCGGCCTGAGAGGGTGAACGG-TAMRA | |||
B. quintana Hypothetical intracellular effector | yopP | F: TAAACCTCGGGGGAAGCAGA | [13] |
R: TTTCGTCCTCAACCCCATCA | |||
FAM-CGTTGCCGACAAGACGTCCTTG-TAMRA | |||
3-oxoacyl-synthase gene | fabF3 | F: GCGGCCTTGCTCTTGATGA | |
R: GCTACTCTGCGTGCCTTGGA | |||
FAM-TGCAGCAGGTGGAGAGAACGTG-TAMRA | |||
Anaplasma spp. 23S ribosomal RNA | TtAna | F: TGACAGCGTACCTTTTGCAT | [28] |
R: TGGAGGACCGAACCTGTTAC | |||
FAM-GGATTAGACCCGAAACCAAG-TAMRA | |||
C. burnetii IS1111 spacer | IS1111 | F: CAAGAAACGTATCGCTGTGGC | [29] |
R: CACAGAGCCACCGTATGAATC | |||
FAM-CCGAGTTCGAAACAATGAGGGCTG-TAMRA |