Molecular detection and identification of tick-borne bacteria and protozoans in goats and wild Siberian roe deer (Capreolus pygargus) from Heilongjiang Province, northeastern China

Table 1 Primers and PCR amplification conditions

Pathogen	Target gene	Primer name	Primer sequence (5′–3′)	Annealing T (°C)	Amplicon size (bp)	References
Piroplasm	18S rRNA	Piro1-S	CTTGACGGTAGGGTATTGGC	55	~ 1410	[24, 25]
		Piro3-AS	CCTTCCTTTAAGTGATAAGGTTCAC	55	~ 1410
		PIRO-A1	CGCAAATTACCCAATCCTGACA	55	~ 430
		PIRO-B	TTAAATACGAATGCCCCCAAC	55	~ 430
A. phagocytophilum	16S rRNA	EE1	CCTGGCTCAGAACGAACGCTGGCGGC	55	~ 1430	[26, 27]
		EE2	AGTCACTGACCCAACCTTAAATGGCTG	55	~ 1430
		SSAP2f	GCTGAATGTGGGGATAATTTAT	60	641
		SSAP2r	ATGGCTGCTTCCTTTCGGTTA	60	641
A. bovis	16S rRNA	EE1	TCCTGGCTCAGAACGAACGCTGGCGGC	55	~ 1430	[26, 27]
		EE2	AGTCACTGACCCAACCTTAAATGGCTG	55	~ 1430
		AB1f	CTCGTAGCTTGCTATGAGAAC	60	551
		AB1r	TCTCCCGGACTCCAGTCTG	60	551
A. marginale	msp4	AmargMSP4Fw	CTGAAGGGGGAGTAATGGG	60	344	[28]
A. marginale	msp4	AmargMSP4Rev	GGTAATAGCTGCCAGAGATTCC	60	344	[28]
A. ovis	msp4	MSP45	GGGAGCTCCTATGAATTACAGAGAATTGTTTAC	55	869	[29]
A. ovis	msp4	MSP43	CCGGATCCTTAGCTGAACAGAATCTTGC	55	869	[29]
A. capra	gltA	Outer-f	GCGATTTTAGAGTGYGGAGATTG	55	1031	[6]
		Outer-r	TACAATACCGGAGTAAAAGTCAA	55	1031	[6]
		Inner-f	TCATCTCCTGTTGCACGGTGCCC	60	594	[22]
		Inner-r	CTCTGAATGAACATGCCCACCCT	60	594	[22]
	16S rRNA	Forward	GCAAGTCGAACGGACCAAATCTGT	58	1261	[30]
	16S rRNA	Reverse	CCACGATTACTAGCGATTCCGACTTC	58	1261	[30]
Ehrlichia spp.	16S rRNA	ECC	AGAACGAACGCTGGCGGCAAGC	60	450	[26]
Ehrlichia spp.	16S rRNA	ECB	CGTATTACCGCGGCTGCTGGCA	60	450	[26]
SFG rickettsiae	OmpA	Rr190.70	ATGGCGAATATTTCTCCAAAA	55	632	[31]
SFG rickettsiae	OmpA	Rr190.701	GTTCCGTTAATGGCAGCATCT	55	632	[31]

ISSN: 1756-3305