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Table 1 Successful PCR and DNA sequencing of host DNA from gnathiid blood meals

From: Host DNA integrity within blood meals of hematophagous larval gnathiid isopods (Crustacea, Isopoda, Gnathiidae)

Post-feeding (h) Stage 2 pranizae Stage 3 pranizae
1st PCRa N 2nd PCRb N 2 1st PCRa N 2nd PCRb N 2
0 100 10 94.7 19 100 1
4 91.7 12 100 1 100 22
8 100 13 95.8 24 95.8 1
12 94.1 17 100 1 90.5 21 100 2
16 90.5 21 100 2 86.4 22 100 3
24 100 14 89.5 19 100 2
48 15.4 13 76.9 11 100 15
72 nd nd nd   87.5 24 100 3
96 nd nd nd   84.6 13 100 2
120 nd nd nd   55 20 100 9
  1. Notes: Percent successful identification of fish host species Hypoplectrus puella, using 50 ng of DNA template extracted from gnathiid blood meals in 1st PCR, at each preservation time interval (post-feeding. in h) for second- and third-stage pranizae. Percent successful identification of host species after increasing DNA template or reamplification of PCR products (2nd PCR), performed only on samples that did not amplify using 50 ng template DNA
  2. aThe percent successful host identification after the first PCR
  3. bThe percent successful host identification after the second PCR
  4. Abbreviations: N, the total number of samples at each time point; N2, number of samples requiring a reamplification of PCR amplicon or increase in template DNA concentration; nd, not determined