Post-feeding (h) | Stage 2 pranizae | Stage 3 pranizae |
|---|
1st PCRa |
N
| 2nd PCRb |
N
2
| 1st PCRa |
N
| 2nd PCRb |
N
2
|
|---|
0 | 100 | 10 | – | – | 94.7 | 19 | 100 | 1 |
4 | 91.7 | 12 | 100 | 1 | 100 | 22 | – | – |
8 | 100 | 13 | – | – | 95.8 | 24 | 95.8 | 1 |
12 | 94.1 | 17 | 100 | 1 | 90.5 | 21 | 100 | 2 |
16 | 90.5 | 21 | 100 | 2 | 86.4 | 22 | 100 | 3 |
24 | 100 | 14 | – | – | 89.5 | 19 | 100 | 2 |
48 | 15.4 | 13 | 76.9 | 11 | 100 | 15 | – | – |
72 | nd | nd | nd | Â | 87.5 | 24 | 100 | 3 |
96 | nd | nd | nd | Â | 84.6 | 13 | 100 | 2 |
120 | nd | nd | nd | Â | 55 | 20 | 100 | 9 |
- Notes: Percent successful identification of fish host species Hypoplectrus puella, using 50Â ng of DNA template extracted from gnathiid blood meals in 1st PCR, at each preservation time interval (post-feeding. in h) for second- and third-stage pranizae. Percent successful identification of host species after increasing DNA template or reamplification of PCR products (2nd PCR), performed only on samples that did not amplify using 50Â ng template DNA
- aThe percent successful host identification after the first PCR
- bThe percent successful host identification after the second PCR
- Abbreviations: N, the total number of samples at each time point; N2, number of samples requiring a reamplification of PCR amplicon or increase in template DNA concentration; nd, not determined
