| 1b | 2b | 3b | Mean ± SD |
---|
DS | 0.23 | 0.24 | 0.31 | 0.26 ± 0.043c |
GUS | 0.90 | 0.95 | 0.97 | 0.95 ± 0.036 |
BC | 1 | 1 | 1 | 1 ± 0.047 |
- aLevel of mRNAs relative to blank control transcripts with 2-△△ct method
- b1, 2, 3, three biological replicates
- cThe DOPAL synthase gene expression in DOPAL synthase-dsRNA treated group was higher compared with gus-dsRNA treated larvae and blank control (one-way ANOVA, P < 0.01)
- Abbreviations: DS, treatment of larvae with dsDOPAL synthase; GUS, treatment of larvae with dsgus RNAs; BC, blank control