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Table 2 Quantitation of the expressions of DOPAL synthasea after RNAi of larvae

From: RNA interference-mediated knockdown of 3, 4-dihydroxyphenylacetaldehyde synthase affects larval development and adult survival in the mosquito Aedes aegypti

  1b 2b 3b Mean ± SD
DS 0.23 0.24 0.31 0.26 ± 0.043c
GUS 0.90 0.95 0.97 0.95 ± 0.036
BC 1 1 1 1 ± 0.047
  1. aLevel of mRNAs relative to blank control transcripts with 2-ct method
  2. b1, 2, 3, three biological replicates
  3. cThe DOPAL synthase gene expression in DOPAL synthase-dsRNA treated group was higher compared with gus-dsRNA treated larvae and blank control (one-way ANOVA, P < 0.01)
  4. Abbreviations: DS, treatment of larvae with dsDOPAL synthase; GUS, treatment of larvae with dsgus RNAs; BC, blank control