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Table 3 Specification of primer pairs used for gene amplification and PCR conditions

From: Species of Characidotrema Paperna & Thurston, 1968 (Monogenea: Dactylogyridae) from fishes of the Alestidae (Characiformes) in Africa: new species, host-parasite associations and first insights into the phylogeny of the genus

Primer nameMarkerSequence (5′–3′)PCR cycling conditionsReference
S1 (Forward)18S-ITS1ATT CCG ATA ACG AAC GAG ACT94 °C for 2 min (39×: 94 °C for 60 s; 53 °C for 60 s; 72 °C for 90 s) 72 °C for 10 min[44]
IR8 (Reverse)GCT AGC TGC GTT CTT CAT CGA[45]
C1 (Forward)28SACC CGC TGA ATT TAA GCA T94 °C for 2 min (39×: 94 °C for 20 s; 56 °C for 30 s; 72 °C for 90 s) 72 °C for 10 min[46]
D2 (Reverse)TGG TCC GTG TTT CAA GAC[46]