Fig. 1

WaF17.12 in vitro killing activity against sporogonic stages of PbCTRPp.GFP (a) and cell viability assay (b). a Numbers of parasites developed by cultural method from gametocytemic mice bloodare reported after 24 h of incubation at 19 °C in the presence of WaUM3 (10⁸ yeast cells/ml), WaF17.12 (10⁸ yeast cells/ml) and PBS (control group). Mean values ± SEM were estimated from triplicate experiments and statistical significance expressed as a P-value (Mann–Whitney test: Control vs WaUM3: U₍₉₎ = 81, Z = 4.101, P = 0.0041; Control vs WaF17.12: U₍₉₎ = 81, Z = 4.101, P = 0.0041). **P < 0.01. b Fluorescence microscope images of ookinetes treated with the WaF17.12-KT⁺ (containing 100 µg/ml KT) or WaUM3-KT⁻ fraction. Data show an abnormal morphology and PI accumulation only in toxin-treated parasites. Scale-bars: 20 µm