Fig. 1

2D gels of excretory–secretory (ES) proteins of fourth-stage T. circumcincta larvae. ES proteins (23 µg of each) were separated by 2D gel electrophoresis and stained with SimplyBlue^TM SafeStain (Invitrogen). Protein spots upregulated in either T. circumcincta L4 mucosal-dwelling ES proteins or T. circumcincta L4 lumen-dwelling ES proteins are numbered and their protein identifications are shown in Table 1. The two lower panels show 3-dimensional images generated from the areas outlined in the dashed boxes on the gels with the relevant spots circled