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Fig. 4 | Parasites & Vectors

Fig. 4

From: Characterisation of a niche-specific excretory–secretory peroxiredoxin from the parasitic nematode Teladorsagia circumcincta

Fig. 4

Immune recognition of rTci-Prx1 using sera from T. circumcincta trickle-infected sheep. Purified recombinant Tci-Prx1 and other recombinant T. circumcincta ES proteins including Tci-MEP-1 and mut Tci-APY-1 (500 ng of each) were resolved by SDS-PAGE and proteins transferred to nitrocellulose membranes. Blots were probed with pooled sera from 7 helminth-naïve six-month-old sheep at either day 0 (prior to T. circumcincta trickle infection); or day 112 (post T. circumcincta trickle infection). Blots shown in a were probed with anti-goat/sheep IgG-HRP secondary antibodies. Blots shown in b were probed with anti-sheep IgA-HRP secondary antibodies. Specifically bound antibodies were visualised using DAB staining. Arrows indicate presence of either Tci-MEP-1 (57 kDa) or mut Tci-APY-1 (38 kDa)

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Parasites & Vectors

ISSN: 1756-3305

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