Fig. 5
Biochemical analysis of wild-type and mutant Tci-Prx1. a SDS-PAGE analysis of purified wild-type (wt) and mutant (mut) Tci-Prx1 (5 µg of each protein/lane) under reducing and non-reducing conditions. Presence of monomer (1-mer, 23.3 kDa) and dimer (2-mer, 46.6 kDa) is indicated. b Peroxidase activity of wt and mut Tci-Prx1. Each reaction contained 40 µM of H2O2 and either wt Tci-Prx1 or mut Tci-Prx1, and diminishment of peroxide was monitored by FOX assay. Each value is the mean ± SEM, n = 5. Significant differences between wt Tci-Prx1 and mut Tci-Prx1 are shown (t-test, t = 6.768, df = 4, P < 0.001)