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Table 1 Et-EmAMA1 selection based on EYFP expression

From: Co-immunization with two recombinant Eimeria tenella lines expressing immunoprotective antigens of E. maxima elicits enhanced protection against E. maxima infection

Generations

% EYFP expression

Selection strategy

1

32.3

Drug

2

78.3

Drug + FACSa

3

93.2

Drug + FACS

4

96.5

Drugb

5

94.7

Drug

6

94.2

–c

  1. aFacilitating recombinant Eimeria parasite selection by pyrimethamine combined with fluorescence-activated cell sorting (FACS)
  2. bStabilization of the recombinant Eimeria population by pyrimethamine
  3. cThe recombinant Eimeria population was stable without selection pressure
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Parasites & Vectors

ISSN: 1756-3305

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