Skip to main content
Fig. 1 | Parasites & Vectors

Fig. 1

From: A combination of antibodies against Bm86 and Subolesin inhibits engorgement of Rhipicephalus australis (formerly Rhipicephalus microplus) larvae in vitro

Fig. 1

Recombinant Bm86 and subolesin and determination of antigen specific antibody titers. a Coomassie staining (left) and Western blot using anti-HIS mouse IgG (right) of purified subolesin (SUB) using a 4–20% Bis-Tris gel. b Anti-SUB antibody titration by sandwich ELISA; recombinant SUB was captured with anti-HIS mouse IgG and pooled SUB vaccinated cow serum was diluted to calculate endpoint titers. End-point titer cut-off (Bmin*2) is indicated by dashed line. c Coomassie staining (left) and Western blot using Bm86 (P. pastoris-produced) specific rabbit IgG (right) of recombinant Bm86. d Anti-Bm86 antibody titration by sandwich ELISA adapted from Trentelman et al. [35]. Bm86 (baculovirus produced) was captured with Bm86 (P. pastoris-produced) specific rabbit IgG and pooled SUB vaccinated cow serum was diluted to calculate endpoint titers. End point titer cut-off (Bmin*2) is indicated by dashed line

Back to article page