Fig. 5

The effect of one-time diluted monospecific antisera against tick antigens and a 1:1 mixture of these antisera on R. australis larval feeding as compared to tick naïve bovine serum. Larvae of R. australis were fed in vitro on 300 µl antisera against Bm86 or SUB diluted with 300 µl naïve bovine serum. Again, 300 µl anti-Bm86 bovine serum was combined with 300 µl anti-SUB bovine serum to assess synergistic effects of both antigens. After 48 h, ticks were visually scored for feeding. Bars represent the inhibition of feeding as compared to the control group, expressed as a percentage. Error bars represent the standard deviation