Table 1 Primers and probe for the conventional PCR, qPCR and LF-RPA
Assay | Primer/probe name | Sequence (5′–3′) | Product size (bp) | References |
|---|---|---|---|---|
PCR | PerkITS-85 | CCGCTTTGTTTGGATCCC | 703 | [31] |
PerkITS-750 | ACATCAGGCCTTCTAATGATG | [31] | ||
qPCR | Q2-F | TCGATGAAGGACGCAACGAA | 291 | Present study |
Q2-R | CTCATTTCTGCGGGGCTACA | |||
LF-RPA | Pits6-F | CGATGAAGGACGCAACGAAGTG | 186 | Present study |
Pits6-R | biotin-CAAGCGGGATACAAAGCATTAGATT | |||
Probe | FAM-CAGAATTCCGTGAACCAGTAGAAATCTCAA CGCA-(THF)-TACTGCACAAAGGGGA-/C3-spacer/ |