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Table 2 Performance of LF-RPA assay versus qPCR and conventional PCR assays for detection of Perkinsus spp

From: Utilization of recombinase polymerase amplification combined with a lateral flow strip for detection of Perkinsus beihaiensis in the oyster Crassostrea hongkongensis

   

PCR assay

qPCR assay

Positive

Negative

Positive

Negative

LF-RPA assay

 

Positive

25 (TP)

7 (FP)

28 (TP)

4 (FP)

 

Negative

4 (FN)

24 (TN)

2 (FN)

26 (TN)

Performance characteristics

McNemar’s test

 

χ2 = 0.167, P = 0.549

χ2 = 0.364, P = 0.687

Kappa

 

K = 0.634, P = 0.000

K = 0.800, P = 0.000

Sensitivity (%)

 

86.2

93.3

Specificity (%)

 

77.4

86.7

Accuracy (%)

 

81.7

90.0

  1. Notes: Sensitivity = TP/(TP + FN) × 100; specificity = TN/(TN+FP) × 100; accuracy = (TP + TN)/(TP + TN + FP + FN) × 100
  2. Abbreviations: LF: lateral flow; RPA: recombinase polymerase amplification; TP, true positive; FP, false positive; FN, false negative; TN, true negative