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Table 2 Performance of LF-RPA assay versus qPCR and conventional PCR assays for detection of Perkinsus spp

From: Utilization of recombinase polymerase amplification combined with a lateral flow strip for detection of Perkinsus beihaiensis in the oyster Crassostrea hongkongensis

   PCR assayqPCR assay
PositiveNegativePositiveNegative
LF-RPA assay Positive25 (TP)7 (FP)28 (TP)4 (FP)
 Negative4 (FN)24 (TN)2 (FN)26 (TN)
Performance characteristicsMcNemar’s test χ2 = 0.167, P = 0.549χ2 = 0.364, P = 0.687
Kappa K = 0.634, P = 0.000K = 0.800, P = 0.000
Sensitivity (%) 86.293.3
Specificity (%) 77.486.7
Accuracy (%) 81.790.0
  1. Notes: Sensitivity = TP/(TP + FN) × 100; specificity = TN/(TN+FP) × 100; accuracy = (TP + TN)/(TP + TN + FP + FN) × 100
  2. Abbreviations: LF: lateral flow; RPA: recombinase polymerase amplification; TP, true positive; FP, false positive; FN, false negative; TN, true negative