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Fig. 2 | Parasites & Vectors

Fig. 2

From: Microbiota and gut ultrastructure of Anisakis pegreffii isolated from stranded cetaceans in the Adriatic Sea

Fig. 2

Representative electron micrographs of third-stage Anisakis pegreffii larva gut. a Pharynx with uniformly dense cuticle (C) lining the pharyngeal lumen (Ph). Thin electron-lucent muscle fibres (asterisk) are radiating from the cuticle. Arrow indicates the multivesicular body (MVB). b Nucleus of pharyngeal cell (N) with prominent nucleolus (Nu), surrounded by patches of granulated material consistent with glycogen. c Detailed view of the pharyngeal cell nucleus with nuclear pores (arrowheads) and crista-type mitochondria in perinuclear space (arrows). d Ventriculus of L3 with fine electron-lucent content in ventricular lumen (Vl). Apical membrane of ventricular cells lacks brush border (curved arrows), while subapically, crista-type mitochondria (arrows) and single Golgi apparatus (Ga) are seen. e Basal part of the ventricular cell, with electron-lucent vesicles and single mitochondria (arrow). Note the small invaginations of the basal membrane with vesicles close to it (curved arrows). f Spindle-shaped enterocytes with basally located nuclei (N). g Intestinal lumen of L3 with two large vesicles circumscribed by double membrane and filled with finely granulated electron-lucent content. Microvilli (Mv) are seen in cross section. Note the subapical cytoplasm filled with numerous electron-dense tubular structures (asterisks). Insert: detailed view of a vesicle inside the intestinal lumen and apical part of two cells separated by tight junction (arrow). Subapically, abundant electron-dense tubular structures are seen. h Basal part of an enterocyte with crista-type mitochondria (arrows), and large, electron-dense vesicles consistent with yolk. Note the elaborate invaginations of the basal membrane. Insert: detailed view of basal membrane invaginations, with electron-dense tubular structures. i Subapical complex junction separating two cells, with mitochondria (arrows) and vesicles adjacent to lateral membranes. Note the small rootles forming a terminal web (long arrows) extending from the basal ends of microvilli (Mv). j Large MVB with a granule, surrounded by multi-layered membranes resembling myelin sheath. k Subapical cytoplasm with mitochondria (arrows), Golgi apparatus (Ga), and granules surrounded by multi-layered membranes (arrowheads). Scale-bars: a, c, e, insert in g, 500 nm; b, d, k, 1 µm; f, 10 µm; g, h, j, 2 µm; i, insert in h, 200 nm

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