Infection and transmission of Cache Valley virus by Aedes albopictus and Aedes aegypti mosquitoes

Parasites & Vectors

Table 1 Average titers of engorged mosquitoes, infection, dissemination, and transmission rates in mosquitoes challenged with CVV

Mosquito species	0 dpi	7 dpi		14 dpi
Mosquito species	Average titers of engorged mosquitoes (logTCID₅₀/ml)	Infection rate (%)^ab	Dissemination rate (%)^c	Infection rate (%)^ab	Dissemination rate (%)^c	Transmission rate (%)^d
Ae. albopictus	3.7 ± 0.6 (n = 11)	69.2 (45/65)	83.3 (25/30)	56.5 (26/46)	100.0 (12/12)	29.6 (8/27)
Ae. aegypti	4.0 ± 0.8 (n=18)	15.2 (10/66)	100 (4/4)	11.0 (9/82)	100 (5/5)	30.0 (3/10)

^aThe infection rate of CVV at 7 and 14 dpi was determined by the isolation of infectious viruses in tissues of dissected mosquitoes or carcasses of whole mosquitoes using Vero76 cells
^bSignificant differences between Ae. albopictus and Ae. aegypti were detected using Chi-square test
^cThe dissemination rate of CVV at 7 and 14 dpi was calculated based on the detection of infectious viruses in secondary tissues (head, wings and legs) of dissected mosquitoes, which were infected with CVV
^dThe transmission rate of CVV was determined by the incidence of positive detection of viral RNA among saliva of infected mosquitoes using nested RT-PCR

ISSN: 1756-3305